Miki N P, Chantler J K
Division of Medical Microbiology, University of British Columbia, Vancouver, Canada.
Clin Exp Rheumatol. 1992 Jan-Feb;10(1):3-12.
Natural rubella has been reported to be associated with a higher incidence of arthropathy than immunisation with rubella vaccine. In addition, the different vaccines (HPV77/DE5, RA27/3, Cendehill) have been shown to vary in their association with joint symptoms in clinical trials. To investigate possible reasons for these differences in arthritogenicity, the susceptibility of human joint tissue to five rubella virus strains (three vaccines and two wt+) has been examined. Human joint tissue in either organ or dispersed cell-culture was infected in vitro and the degree of replication and persistence of each rubella strain compared. The wt+ strains (M33 and Therien) replicated to high titre in both cell and organ cultures and persisted for over 2 months. The HPV77/DE5 strain (Meruvax I) showed a very similar pattern. In contrast, the replication of RA27/3 (Meruvax II) and Cendehill (Cendevax) was highly restricted in joint cells and both of these strains showed very limited ability to penetrate and persist in the organ cultures. These results concur with the differences in arthritogenicity observed between the strains in vivo, suggesting that local viral replication may play a role in the pathogenesis of rubella-associated arthritis.
据报道，自然感染风疹比接种风疹疫苗引发关节病的发病率更高。此外，不同的疫苗（HPV77/DE5、RA27/3、Cendehill）在临床试验中与关节症状的关联有所不同。为了探究这些致关节炎性差异的可能原因，研究人员检测了人类关节组织对五种风疹病毒株（三种疫苗株和两种野生型毒株）的易感性。将人类关节组织以器官或分散细胞培养的形式进行体外感染，并比较每种风疹毒株的复制程度和持续性。野生型毒株（M33和Therien）在细胞和器官培养中均能复制至高滴度，并持续超过2个月。HPV77/DE5毒株（默克瓦克斯I）表现出非常相似的模式。相比之下，RA27/3（默克瓦克斯II）和Cendehill（森德瓦克斯）在关节细胞中的复制受到高度限制，并且这两种毒株在器官培养中的穿透和持续能力都非常有限。这些结果与体内观察到的毒株致关节炎性差异一致，表明局部病毒复制可能在风疹相关性关节炎的发病机制中起作用。
