体外铁钼辅因子合成的核苷酸和二价阳离子特异性
Nucleotide and divalent cation specificity of in vitro iron-molybdenum cofactor synthesis.
作者信息
Chatterjee R, Allen R M, Shah V K, Ludden P W
机构信息
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.
出版信息
J Bacteriol. 1994 May;176(9):2747-50. doi: 10.1128/jb.176.9.2747-2750.1994.
The nucleotide and divalent cation requirements of the in vitro iron-molybdenum cofactor (FeMo-co) synthesis system have been compared with those of substrate reduction by nitrogenase. The FeMo-co synthesis system specifically requires ATP, whereas both 1,N6-etheno-ATP and 2'-deoxy-ATP function in place of ATP in substrate reduction (M. F. Weston, S. Kotake, and L. C. Davis, Arch. Biochem. Biophys. 225:809-817, 1983). Mn2+, Ca2+, and Fe2+ substitute for Mg2+ to various extents in in vitro FeMo-co synthesis, whereas Ca2+ is ineffective in substrate reduction by nitrogenase. The observed differences in the nucleotide and divalent cation specificities suggest a role(s) for the nucleotide and divalent cation in in vitro FeMo-co synthesis that is distinct from their role(s) in substrate reduction.
已将体外铁钼辅因子（FeMo-co）合成系统对核苷酸和二价阳离子的需求与固氮酶进行底物还原时的需求作了比较。FeMo-co合成系统特别需要ATP，而在底物还原过程中，1,N6-乙烯基ATP和2'-脱氧ATP均可替代ATP发挥作用（M. F. 韦斯顿、S. 小竹和L. C. 戴维斯，《生物化学与生物物理学报》225:809 - 817, 1983）。在体外FeMo-co合成中，Mn2+、Ca2+和Fe2+在不同程度上可替代Mg2+，而Ca2+对固氮酶进行底物还原无效。在核苷酸和二价阳离子特异性方面观察到的差异表明，核苷酸和二价阳离子在体外FeMo-co合成中的作用与其在底物还原中的作用不同。
Zotero 插件