嗜热栖热菌（Thermus thermophilus）基因在大肠杆菌细胞中的过表达。
Overexpression of genes of an extreme thermophile Thermus thermophilus, in Escherichia coli cells.
作者信息
Ishida M, Oshima T
机构信息
Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
出版信息
J Bacteriol. 1994 May;176(9):2767-70. doi: 10.1128/jb.176.9.2767-2770.1994.
Abstract
The 3-isopropylmalate dehydrogenase gene from an extreme thermophile, Thermus thermophilus, was not expressed in Escherichia coli unless a palindromic structure around the ribosome binding site was eliminated or a leader open reading frame was introduced into the upstream flanking region of the gene. This report suggests a way to increase the expression of this gene, with a high G+C content, in E. coli.
摘要
来自嗜热栖热菌（Thermus thermophilus）这种极端嗜热菌的3-异丙基苹果酸脱氢酶基因，在大肠杆菌中无法表达，除非核糖体结合位点周围的回文结构被消除，或者在该基因的上游侧翼区域引入一个前导开放阅读框。本报告提出了一种在大肠杆菌中提高这个高G+C含量基因表达的方法。
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本文引用的文献
1
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J Biochem. 1981 Feb;89(2):677-82. doi: 10.1093/oxfordjournals.jbchem.a133245.
2
Cloning and expression of the leucine gene from Thermus thermophilus in Escherichia coli.嗜热栖热菌亮氨酸基因在大肠杆菌中的克隆与表达。
Gene. 1980 Jul;10(2):137-45. doi: 10.1016/0378-1119(80)90131-6.
3
Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325.新型克隆载体的构建与特性分析。IV. pBR322和pBR325的缺失衍生物
Gene. 1980 May;9(3-4):287-305. doi: 10.1016/0378-1119(90)90328-o.
4
Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information.利用热力学和辅助信息对大型RNA序列进行最优计算机折叠
Nucleic Acids Res. 1981 Jan 10;9(1):133-48. doi: 10.1093/nar/9.1.133.
5
Lysis gene of bacteriophage MS2 is activated by translation termination at the overlapping coat gene.噬菌体MS2的裂解基因通过重叠衣壳基因处的翻译终止而被激活。
J Mol Biol. 1987 Jun 5;195(3):517-24. doi: 10.1016/0022-2836(87)90180-x.
6
Translation of a synthetic two-cistron mRNA in Escherichia coli.一种合成双顺反子mRNA在大肠杆菌中的翻译。
Proc Natl Acad Sci U S A. 1986 Nov;83(22):8506-10. doi: 10.1073/pnas.83.22.8506.
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J Biochem. 1987 May;101(5):1281-8. doi: 10.1093/oxfordjournals.jbchem.a121992.
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