嗜热细菌的亮氨酸基因在大肠杆菌中的表达。
Expression of leucine genes from an extremely thermophilic bacterium in Escherichia coli.
作者信息
Croft J E, Love D R, Bergquist P L
机构信息
Cell Biology Department, University of Auckland, New Zealand.
出版信息
Mol Gen Genet. 1987 Dec;210(3):490-7. doi: 10.1007/BF00327202.
The organisation of the leucine genes in Thermus thermophilus HB8 was analysed by examining the ability of recombinant DNAs to complement Escherichia coli mutations. The arrangement of the genes is different from that in the mesophilic bacteria E. coli and Salmonella typhimurium. The promoter responsible for the expression of the leuB, leuC and leuD genes of Thermus HB8 in E. coli was identified. The sequence of Thermus DNA containing this promoter revealed structural similarities to the promoter and attenuator regions of the E. coli leucine operon.
通过检测重组DNA对大肠杆菌突变体的互补能力，分析了嗜热栖热菌HB8中亮氨酸基因的组织方式。这些基因的排列与嗜温菌大肠杆菌和鼠伤寒沙门氏菌中的不同。鉴定出了负责嗜热栖热菌HB8的leuB、leuC和leuD基因在大肠杆菌中表达的启动子。包含该启动子的嗜热栖热菌DNA序列显示出与大肠杆菌亮氨酸操纵子的启动子和弱化子区域的结构相似性。
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