Arora D J, Henrichon M
Virology Research Center, Institut Armand Frappier, Université du Québec, Laval, Canada.
J Infect Dis. 1994 May;169(5):1129-33. doi: 10.1093/infdis/169.5.1129.
There are conflicting reports regarding superoxide anion (O2-) production by human polymorphonuclear leukocytes (PMNL) that have been activated by influenza virus. In the present study, the output of O2- was determined by measuring superoxide dismutase-inhibitable cytochrome c reduction. Incubation of PMNL with purified influenza matrix (M) protein, neuraminidase (NA), or hemagglutinin (HA) enhanced the production of O2-: 4.93 nmol of O2-/4 x 10(5) cells/15 min was produced with M protein, 5.20 with NA, and 6.89 with HA. These values were significantly higher (P < .05) than that for untreated PMNL (1.51). Both nonglycosylated and glycosylated proteins had the potential to generate O2- in human PMNL. Neither the hemagglutinating activity of HA nor the enzymatic activity of NA were necessary for viral protein activation of PMNL.
关于流感病毒激活的人多形核白细胞（PMNL）产生超氧阴离子（O2-），有相互矛盾的报道。在本研究中，通过测量超氧化物歧化酶抑制的细胞色素c还原作用来确定O2-的产量。用纯化的流感病毒基质（M）蛋白、神经氨酸酶（NA）或血凝素（HA）孵育PMNL可增强O2-的产生：M蛋白产生4.93 nmol O2-/4×10（5）个细胞/15分钟，NA产生5.20，HA产生6.89。这些值显著高于未处理的PMNL（1.51）（P<0.05）。非糖基化和糖基化蛋白都有在人PMNL中产生O2-的潜力。HA的血凝活性和NA的酶活性对于PMNL的病毒蛋白激活都不是必需的。
