Morsy T A, Abdel Mawla M M, Salama M M, Hamdi K N
Department of Parasitology, Faculty of Medicine, Ain Shams University, Abbassia, Cairo, Egypt.
J Egypt Soc Parasitol. 1994 Apr;24(1):85-91.
Intact cystozoites extracted from naturally infected bovine flesh were used as an antigen to develop an ELISA for serodiagnosing sarcocystosis. The antigen appeared non- reactive with known non specific rat sera. Comparative testing of intact and sonicated antigens using known positive and control rat sera showed the former to be more sensitive in detecting specific antibodies at high serum dilutions. Thus, ELISA using intact cystozoites seems promising as a screening method for the epidemiological investigations of the infection.
从自然感染的牛肌肉中提取的完整囊合子被用作抗原，以开发一种用于血清学诊断肉孢子虫病的酶联免疫吸附测定法（ELISA）。该抗原与已知的非特异性大鼠血清无反应。使用已知的阳性和对照大鼠血清对完整抗原和超声处理抗原进行的对比测试表明，在高血清稀释度下检测特异性抗体时，前者更敏感。因此，使用完整囊合子的ELISA作为该感染流行病学调查的筛查方法似乎很有前景。
