Connick K, Lalor R, Murphy A, O'Neill S M, El Shanawany Eman E
1School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.
2Department of Parasitology and Animal Diseases, National Research Centre, El Bhooth St, Dokki, Post Pox 12622, Giza, Egypt.
J Parasit Dis. 2020 Mar;44(1):186-193. doi: 10.1007/s12639-019-01181-9. Epub 2019 Nov 28.
a coccidian tissue parasite that causes infection in buffalo in countries such an Egypt, China, Iraq and Iran, resulting in significant economic losses to the agricultural industry annually. There is a lack of studies examining host-parasite interactions at the level of the immune response and the present study investigates the interaction between whole cyst antigens (SFWCA) and dendritic cells (DCs), cells critical to the activation of adaptive immunity. In this study bone marrow derived DCs (BMDCs) were phenotyped following treatment with SFWCA by measuring cell viability, cytokine secretion, and cell surface marker expression. While SFWCA exhibited cytotoxic effects on BMDCs at higher concentrations, lower concentrations of SFWCA activated pro-inflammatory DCs that significantly secreted interleukin (IL)-12p40, tumor necrosis factor alpha, IL-6 and IL-10. These cells also displayed enhanced expression of TLR4, CD80, CD86 and MHC II on their surface, which is indicative of full DCs maturation. Moreover, SFWCA significantly attenuated the capacity of BMDCs to suppress Th2 associated cytokines, notably IL-5 and IL-13, while simultaneously exhibiting no effects on the secretion of interferon (IFN)-γ, IL-2, IL-17, and IL-10. In conclusion, this is the first study to provide fundamental insight into the activation of DCs by SFWCA, providing us with some awareness into the interaction of the parasite with its host. The pro-inflammatory inducing ability of this antigen is in keeping with studies performed in other protozoan parasites and therefore understanding these interactions is important in the development of future therapeutic strategies.
一种球虫组织寄生虫，在埃及、中国、伊拉克和伊朗等国家导致水牛感染，每年给农业产业造成重大经济损失。目前缺乏在免疫反应层面研究宿主 - 寄生虫相互作用的相关研究，本研究调查了全囊抗原（SFWCA）与树突状细胞（DCs）之间的相互作用，树突状细胞是激活适应性免疫的关键细胞。在本研究中，通过测量细胞活力、细胞因子分泌和细胞表面标志物表达，对经SFWCA处理后的骨髓来源树突状细胞（BMDCs）进行表型分析。虽然高浓度的SFWCA对BMDCs表现出细胞毒性作用，但低浓度的SFWCA激活了促炎性DCs，这些细胞显著分泌白细胞介素（IL）-12p40、肿瘤坏死因子α、IL-6和IL-10。这些细胞表面还显示出TLR4、CD80、CD86和MHC II的表达增强，这表明DCs完全成熟。此外，SFWCA显著削弱了BMDCs抑制Th2相关细胞因子（特别是IL-5和IL-13）的能力，同时对干扰素（IFN）-γ、IL-2、IL-17和IL-10的分泌没有影响。总之，这是第一项深入研究SFWCA激活DCs的研究，使我们对该寄生虫与其宿主之间的相互作用有了一定认识。这种抗原的促炎诱导能力与在其他原生动物寄生虫中进行的研究一致，因此了解这些相互作用对于未来治疗策略的开发很重要。
