Yamagishi M, Ishizuka Y
Department of Otolaryngology, Mizonokuchi Hospital, Teikyo University School of Medicine, Tokyo.
Nihon Jibiinkoka Gakkai Kaiho. 1994 Mar;97(3):466-72. doi: 10.3950/jibiinkoka.97.466.
Abnormal tau protein expression was immunohistochemically examined in biopsied human olfactory mucosa. The olfactory mucosa of 25 patients with olfactory disturbances, whose structures were relatively preserved, was immunostained with a polyclonal antibody derived from neurofibrillary tangles from the brains of patients with Alzheimer's disease. Abnormal tau protein immunoreactivity was found in the olfactory vesicles, dendrites and perikarya of the olfactory receptor cells in the epithelium and olfactory nerve bundles in the lamina propria. This immunoreactivity in the olfactory nerve bundles was observed in patients in their teens to 80's, and the immunoreactivity of the olfactory vesicles and dendrites of the olfactory receptor cells was widely distributed in patients in their 30's to 70's. On the other hand, the immunoreactivity of the perikarya of the olfactory receptor cells was observed only in patients in their 50's and 60's. These results indicate that abnormal tau protein accumulation of perikarya is a pathological change and is related to aging of the olfactory neurons.
通过免疫组织化学方法检测活检的人类嗅黏膜中异常tau蛋白的表达。对25例嗅觉障碍患者的嗅黏膜进行检测，这些患者的嗅黏膜结构相对保存完好，用源自阿尔茨海默病患者大脑神经原纤维缠结的多克隆抗体进行免疫染色。在上皮中的嗅泡、嗅受体细胞的树突和胞体以及固有层中的嗅神经束中发现了异常的tau蛋白免疫反应性。在十几岁到八十多岁的患者中均观察到嗅神经束中的这种免疫反应性，而嗅受体细胞的嗅泡和树突的免疫反应性在三十多岁到七十多岁的患者中广泛分布。另一方面，仅在五十多岁和六十多岁的患者中观察到嗅受体细胞胞体的免疫反应性。这些结果表明，嗅受体细胞胞体中异常tau蛋白的积累是一种病理变化，并且与嗅神经元的衰老有关。
