Pratt H L, Tanaka S
National Oceanic and Atmospheric Administration, National Marine Fisheries Service, Narragansett Laboratory, Rhode Island 02882-1199.
J Morphol. 1994 Mar;219(3):297-308. doi: 10.1002/jmor.1052190309.
Two basic types of spermatozoan aggregates, spermatophores and spermatozeugmata, found in 14 different species of sharks, one species of skate, and one species of chimaera (holocephalan), were investigated using light and scanning electron microscopy. Spermatophores, aggregates (usually 1,000-6,000 microns in diameter and larger) of randomly clumped sperm embedded in and surrounded by an eosinophilic matrix, were found in Alopias superciliosus, Odontaspis taurus, Carcharodon carcharias, Isurus oxyrinchus, and Lamna nasus. Three types of spermatozeugmata, sperm structures without a surrounding capsule or matrix, are described. The first, clumps of 60-200 sperm unbound in a supporting matrix, are found in Squalus acanthias and Hydrolagus colliei. In the second type, single-layered spheres are formed of sperm clumps with the sperm heads bound in a common supporting matrix. These are found in Carcharhinus limbatus and Carcharhinus plumbeus. The third type of spermatozeugmata are large multilayered, compound structures formed by the accretion of several single-layered aggregates. These multilayered structures characteristically are found in Carcharhinus falciformis, C. limbatus, Carcharhinus obscurus, C. plumbeus, Carcharhinus porosus, Prionace glauca, Rhizoprionodon terraenovae, and Sphyrna lewini. Sperm aggregates of all types are stored between the septa and in the lumen of the terminal ampulla of the epididymis. In their various forms they are the final product of the mature male elasmobranch reproductive tract. In a male with mature claspers, the presence of sperm aggregates is a more reliable indicator of maturity and sexual activity than is clasper condition alone.
利用光学显微镜和扫描电子显微镜对在14种不同鲨鱼、1种鳐鱼和1种银鲛（全头亚纲）中发现的两种基本类型的精子聚集体——精荚和精子束进行了研究。精荚是随机聚集的精子形成的聚集体（直径通常为1000 - 6000微米及更大），包埋于嗜酸性基质中并被其包围，在大眼长尾鲨、沙虎鲨、大白鲨、尖吻鲭鲨和长鳍鲔中被发现。文中描述了三种类型的精子束，即没有周围包膜或基质的精子结构。第一种是60 - 200个精子聚集在一起且无支撑基质束缚，见于棘角鲨和银鲛。第二种类型是由精子团形成的单层球体，精子头部束缚于共同的支撑基质中。这在黑边真鲨和铅灰真鲨中被发现。第三种类型的精子束是由几个单层聚集体堆积形成的大型多层复合结构。这些多层结构典型地见于镰状真鲨、黑边真鲨、朦胧真鲨、铅灰真鲨、多孔真鲨、灰鲭鲨、窄头双髻鲨和路氏双髻鲨。所有类型的精子聚集体都储存于附睾末端壶腹的隔膜之间和管腔内。它们以各种形式存在，是成熟雄性板鳃亚纲动物生殖道的最终产物。在具有成熟交合突的雄性中，精子聚集体的存在比单独的交合突状况更能可靠地指示成熟度和性活动。
