Chávez-Cartaya R E, Ramirez-Romero P, Calne R Y, Jamieson N V
Department of Surgery, Addenbrookes Hospital, University of Cambridge Clinical School, United Kingdom.
J Surg Res. 1994 May;56(5):473-7. doi: 10.1006/jsre.1994.1075.
Estimation of blood flow in the microcirculation is essential in the analysis of the events that occur during organ ischemia and subsequent reperfusion. In this study the use of laser-Doppler flowmetry is evaluated as a method for studying liver blood flow in the rat using an in vivo ischemia-reperfusion model. The experimental procedure consists of the temporary interruption of blood flow to the left lateral and medial lobes of the liver and subsequent recording of the postischemic liver blood flow using a laser-Doppler flowmeter. Flow was recorded for 60 min after periods of ischemia of 30 sec (control), 30, 45, and 60 min. The reperfusion records showed a biphasic curve with a mean flow of 59.6% of the baseline after 30 min of ischemia and of 38.3 and 41.1% after 45 and 60 min of ischemia, respectively. There were statistically significant differences between all the groups and the controls, and between 45 and 30 min of ischemia but not between 45 and 60 min. This is a useful model for studying ischemic liver injury in the rat.
微循环中血流的估测对于分析器官缺血及随后再灌注过程中发生的事件至关重要。在本研究中，使用体内缺血-再灌注模型，对激光多普勒血流仪作为研究大鼠肝脏血流的一种方法进行了评估。实验过程包括暂时中断肝脏左外侧叶和内侧叶的血流，随后使用激光多普勒血流仪记录缺血后肝脏血流。在30秒（对照）、30、45和60分钟的缺血期后记录60分钟的血流。再灌注记录显示为双相曲线，缺血30分钟后平均血流为基线的59.6%，缺血45和60分钟后分别为38.3%和41.1%。所有组与对照组之间，以及缺血45分钟和30分钟之间存在统计学显著差异，但缺血45分钟和60分钟之间无差异。这是研究大鼠缺血性肝损伤的一个有用模型。
