Chávez-Cartaya R E, Ramirez P, Jamieson N V
Department of Surgery, Addenbrookes Hospital, University of Cambridge Clinical School, UK.
Eur Surg Res. 1995;27(2):82-92. doi: 10.1159/000129377.
Tissue oxygenation depends on the volume of oxygen consumed by the tissue and the volume of oxygen supply. This is particularly important in the liver after ischaemia and reperfusion, due to the relatively low oxygen saturation of the portal blood flow, the main source of oxygen to the liver. In this study we established a correlation between the postischaemic liver blood flow and tissue haemoglobin saturation, measured by laser Doppler flowmetry and laser surface photometry, with the purpose of investigating the possible role of a postischaemic imbalance of oxygen delivery/uptake in reperfusion injury. The experimental procedure consisted of the temporary interruption of blood flow to the left lateral and medial lobes of the rat liver in vivo, and subsequent reperfusion after defined periods, recording the postischaemic liver blood flow and liver oxyhaemoglobin saturation. Changes were found in the postischaemic liver blood flow and haemoglobin saturation in all the groups when compared to control values, showing a correlation between the length of the period of ischaemia and the magnitude of the alteration in the reperfusion blood flow and oxygenation. These alterations may be considered as a prolongation of the metabolic condition of ischaemia and may be part of an additional tissue damage upon reperfusion.
组织氧合取决于组织消耗的氧量和供氧量。这在肝脏缺血再灌注后尤为重要，因为门静脉血流是肝脏主要的氧供来源，其氧饱和度相对较低。在本研究中，我们通过激光多普勒血流仪和激光表面光度法测量，建立了缺血后肝脏血流与组织血红蛋白饱和度之间的相关性，目的是研究缺血后氧输送/摄取失衡在再灌注损伤中可能发挥的作用。实验过程包括在体内暂时阻断大鼠肝脏左外叶和中叶的血流，在规定时间后进行再灌注，记录缺血后肝脏血流和肝脏氧合血红蛋白饱和度。与对照值相比，所有组的缺血后肝脏血流和血红蛋白饱和度均有变化，表明缺血时间长短与再灌注血流和氧合变化程度之间存在相关性。这些改变可被视为缺血代谢状态的延长，可能是再灌注时额外组织损伤的一部分。
