Klefstrom J, Franssila K, Peltomäki P, Kaartinen M, Solin M L, Knuutila S
Department of Pathology, University of Helsinki, Finland.
Leuk Res. 1994 Apr;18(4):245-50. doi: 10.1016/0145-2126(94)90026-4.
The frequencies of major breakpoint region (MBR) and minor cluster region (MCR) breakpoint sites of t(14;18) were examined by polymerase chain reaction and Southern blotting in 50 non-Hodgkin's lymphomas with cytogenetic evidence of t(14;18). A translocation breakpoint was detected in 41 cases (82%). The MBR was involved in 66%, and the MCR in 16% of the cases. Most cases in the present series were lymphomas with a follicular or diffuse growth pattern, 38 being of the centroblastic/centrocytic type and eight of the centroblastic type. The series also included four lymphomas of probable non-follicular center cell origin. MBR and/or MCR breakpoints were found in all studied lymphoma subtypes and in the majority of these most of the breakpoints were in the MBR and a minor portion of the breakpoints in the MCR. Our results suggest that a breakpoint site is not related to growth pattern or neoplastic cell type in follicular center cell lymphomas with t(14;18).
采用聚合酶链反应和Southern印迹法，对50例有细胞遗传学证据显示t(14;18)的非霍奇金淋巴瘤患者的主要断裂点区域(MBR)和次要簇集区域(MCR)的断裂点位点频率进行了检测。在41例(82%)患者中检测到易位断裂点。66%的病例累及MBR，16%的病例累及MCR。本系列中的大多数病例为具有滤泡性或弥漫性生长模式的淋巴瘤，其中38例为中心母细胞/中心细胞型，8例为中心母细胞型。该系列还包括4例可能起源于非滤泡中心细胞的淋巴瘤。在所有研究的淋巴瘤亚型中均发现了MBR和/或MCR断裂点，并且在这些亚型中的大多数中，大多数断裂点位于MBR，一小部分断裂点位于MCR。我们的结果表明，在伴有t(14;18)的滤泡中心细胞淋巴瘤中，断裂点位点与生长模式或肿瘤细胞类型无关。
