Goto M, Oshima I, Hasegawa M, Ebihara S
Department of Animal Physiology, Faculty of Agriculture, Nagoya University, Japan.
Brain Res Mol Brain Res. 1994 Feb;21(3-4):349-54. doi: 10.1016/0169-328x(94)90266-6.
Melatonin is synthesized from serotonin by the enzymes serotonin N-acetyltransferase (SNAT) and hydroxyindole-O-methyl-transferase (HIOMT). We have previously reported that C57BL/6 mice do not have SNAT activity because of a mutation in an autosomal gene which is responsible for the absence of normal SNAT activity. In the present study, we have tried to map the loci of Nat-2 (the locus controlling SNAT activity) on chromosomes using a set of the BxH recombinant inbred strains which were derived from an initial cross between C3H/He with SNAT and C57BL/6 without the enzyme. Based on strain distribution patterns (SDPs), a close linkage on chromosome 11 was found between Nat-2, Es-3 (esterase-3), Glk (the locus controlling galactokinase activity) and Myla (myosin alkali light chains expressed in cardiac atrial muscle). The linkage between Nat-2 and Es-3 was confirmed by a conventional linkage test and the recombination frequency between these loci was estimated to be 16.1 +/- 3.6% (mean +/- S.E.M.).
褪黑素由色氨酸经色氨酸N - 乙酰基转移酶（SNAT）和羟基吲哚 - O - 甲基转移酶（HIOMT）合成。我们之前报道过，C57BL/6小鼠由于常染色体基因突变而不具有SNAT活性，该突变导致正常SNAT活性缺失。在本研究中，我们试图利用一组BxH重组近交系来定位染色体上Nat - 2（控制SNAT活性的基因座）的位点，这些重组近交系源自C3H/He（具有SNAT）与C57BL/6（无该酶）的初始杂交。基于品系分布模式（SDPs），发现Nat - 2、Es - 3（酯酶 - 3）、Glk（控制半乳糖激酶活性的基因座）和Myla（在心房肌中表达的肌球蛋白碱性轻链）在11号染色体上紧密连锁。通过传统连锁试验证实了Nat - 2和Es - 3之间的连锁关系，估计这些基因座之间的重组频率为16.1 +/- 3.6%（平均值 +/- 标准误）。
