Honer W G, Beach T G, Hu L, Berry K, Dorovini-Zis K, Moore G R, Woodhurst B
Department of Psychiatry, University of British Columbia, Vancouver, Canada.
Acta Neuropathol. 1994;87(2):202-10. doi: 10.1007/BF00296191.
Immunostaining of synaptic terminals was studied in the hippocampus of 26 patients who had surgical resections for intractable temporal lobe epilepsy. Two monoclonal antibodies (EP10 and SP12) reactive with distinct synaptic antigens were used on paraffin-embedded tissues. The results indicated qualitative reductions on synaptic terminals in CA4 and other regions where cell loss is reported. The inner molecular layer of the dentate gyrus was observed to have increased synaptic immunostaining. Synaptic terminal loss in CA4 and redistribution in the molecular layer were most frequent in cases with hippocampal sclerosis. However, both forms of synaptic pathology were also noted in most cases where the pathological findings were classified as indefinite, and in some cases associated with mass lesions of the temporal lobe. These results support the importance of neuronal loss and synaptic reorganization as possible mechanisms of illness in epilepsy. They also indicate that synaptic immunostaining may be a useful adjunct to routine neuropathological diagnostic techniques.
对26例因顽固性颞叶癫痫接受手术切除的患者海马中的突触终末进行了免疫染色研究。使用两种与不同突触抗原反应的单克隆抗体（EP10和SP12）对石蜡包埋组织进行检测。结果表明，在报告有细胞丢失的CA4区和其他区域，突触终末出现了质性减少。观察到齿状回的内分子层突触免疫染色增加。CA4区的突触终末丢失和分子层的重新分布在海马硬化病例中最为常见。然而，在大多数病理结果分类为不明确的病例中，以及在一些与颞叶占位性病变相关的病例中，也都发现了这两种形式的突触病理改变。这些结果支持了神经元丢失和突触重组作为癫痫发病可能机制的重要性。它们还表明，突触免疫染色可能是常规神经病理学诊断技术的有用辅助手段。
