Bessler H, Notti I, Cohen A M, Klein B, Djaldetti M
Department of Medicine B, Hasharon Hospital, Golda Medical Center, Petah Tiqva, Israel.
Am J Hematol. 1994 Jun;46(2):65-71. doi: 10.1002/ajh.2830460202.
An attempt was made to clarify the mechanism by which splenic irradiation in patients with B-chronic lymphocytic leukemia (B-CLL) can induce a reduction in lymph node size. For this purpose peripheral blood lymphocytes from B-CLL patients were exposed to cobalt irradiation and were cultured for 1-8 days. The effect of the supernatants on the proliferation capacity of normal and malignant human cells was examined. A suppression of phytohemagglutinin (PHA)-induced proliferation of autologous and heterologous B-CLL lymphocytes was observed, whereas there was no effect on the proliferation of lymphocytes obtained from healthy volunteers. In addition, supernatants of irradiated B-CLL lymphocytes inhibited thymidine incorporation into blasts derived from patients with acute leukemia and the lymphoblastoid cell line Daudi, but they did not exert any effect on normal cells obtained from human embryonic liver. These results suggest the secretion of some factor(s) by irradiated B-CLL lymphocytes, which may inhibit the proliferation of malignant cells but has no effect on normal cells.
人们试图阐明B慢性淋巴细胞白血病（B-CLL）患者脾脏照射可导致淋巴结大小减小的机制。为此，将B-CLL患者的外周血淋巴细胞暴露于钴照射下，并培养1至8天。检测了上清液对正常和恶性人类细胞增殖能力的影响。观察到植物血凝素（PHA）诱导的自体和异源B-CLL淋巴细胞增殖受到抑制，而对健康志愿者的淋巴细胞增殖没有影响。此外，照射后的B-CLL淋巴细胞上清液抑制胸苷掺入急性白血病患者和淋巴母细胞系Daudi的母细胞中，但对人胚胎肝来源的正常细胞没有任何影响。这些结果表明，照射后的B-CLL淋巴细胞分泌了某些因子，这些因子可能抑制恶性细胞的增殖，但对正常细胞没有影响。
