巴斯德梭菌亮氨酸生物合成途径中相关基因的核苷酸序列。

The nucleotide sequence of genes involved in the leucine biosynthetic pathway of Clostridium pasteurianum.

作者信息

Oultram J D, Loughlin M, Walmsley R, Gunnery S M, Minton N P

机构信息

Molecular Genetics Group, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire, UK.

出版信息

DNA Seq. 1993;4(2):105-11. doi: 10.3109/10425179309020149.

DOI:10.3109/10425179309020149

PMID:8173074

Abstract

A 2.2 kb SphI/ClaI fragment of the Clostridium pasteurianum chromosome has previously been cloned and shown to complement leuB401 and leuC171 mutations in Escherichia coli. The nucleotide sequence of this fragment has been determined (2327 bp) and carries three open reading frames. The products of translation of these reading frames display significant homologies with the alpha-isopropylmalate isomerase subunit (leuD) gene of Salmonella typhimurium, the beta-isopropylmalate dehydrogenase (leuB) genes of several organisms, and the dihydroxyacid dehydrase (ilvD) gene of E. coli.

摘要

先前已克隆了巴氏芽孢杆菌染色体的一个2.2 kb SphI/ClaI片段，并证明其可互补大肠杆菌中的leuB401和leuC171突变。已确定该片段的核苷酸序列（2327 bp），并带有三个开放阅读框。这些阅读框的翻译产物与鼠伤寒沙门氏菌的α-异丙基苹果酸异构酶亚基（leuD）基因、几种生物的β-异丙基苹果酸脱氢酶（leuB）基因以及大肠杆菌的二羟基酸脱水酶（ilvD）基因具有显著同源性。