Conner M K, Boggs S S, Turner J H
Chromosoma. 1978 Sep 11;68(4):303-11. doi: 10.1007/BF00327165.
BrdU and BrdC have been employed as DNA labeling agents for differentiation of sister chromatids and for extension of sister chromatid exchange (SCE) methods to regenerating murine liver cells in vivo. Comparisons were made between bone marrow and liver cells isolated simultaneously from mice following DNA labeling with either BrdC or BrdU. Although the total mitotic yield of bone marrow cells was considerably greater than in liver, a higher percentage of second division metaphases was observed in liver cell preparations. The percentages of second division c-metaphase cells observed were 31.5% in bone marrow and 73% in liver cell preparations. Utilizing either BrdU or BrdC, no significant difference in percentage of second division metaphases was discerned. The number of spontaneous SCEs per cell was distributed according to the Poisson probability function. No significant differences in mean numbers of SCEs per cell were found in comparisons of bone marrow (1.40) and liver cells (1.65) or of cells which had incorporated BrdU or BrdC.
5-溴脱氧尿嘧啶核苷（BrdU）和5-溴脱氧胞嘧啶核苷（BrdC）已被用作DNA标记剂，用于区分姐妹染色单体，并将姐妹染色单体交换（SCE）方法扩展到体内再生的小鼠肝细胞。在用BrdC或BrdU进行DNA标记后，对同时从小鼠分离的骨髓细胞和肝细胞进行了比较。虽然骨髓细胞的总有丝分裂产量比肝细胞高得多，但在肝细胞制剂中观察到更高百分比的第二次分裂中期。观察到的第二次分裂c-中期细胞百分比在骨髓中为31.5%，在肝细胞制剂中为73%。使用BrdU或BrdC，第二次分裂中期的百分比没有显著差异。每个细胞的自发SCE数量根据泊松概率函数分布。在比较骨髓细胞（1.40）和肝细胞（1.65）或掺入BrdU或BrdC的细胞时，未发现每个细胞的SCE平均数有显著差异。
