Shi G, Weh H J, Hossfeld D K
Department of Oncology/Hematology, Medical University Clinic, Hamburg, Germany.
Cancer Genet Cytogenet. 1994 Mar;73(1):17-22. doi: 10.1016/0165-4608(94)90177-5.
In 10 patients with various hematopoietic disorders, i(17q) has been studied by the technique of fluorescence in situ hybridization (FISH) both in metaphase and interphase cells using an alpha-satellite centromere-specific probe #17. We observed some minor quantitative differences between metaphase and interphase cells with regard to the incidence of i(17q) in individual cases and considerable heteromorphism of the size of signal of i(17q) among different cases. Our results suggest that formation of i(17q) results from a break in the p-arm proximal to the centromere and that the breakpoints vary from case to case.
在10例患有各种造血系统疾病的患者中，使用α-卫星着丝粒特异性探针#17，通过荧光原位杂交（FISH）技术，在中期和间期细胞中对i(17q)进行了研究。我们观察到，在个别病例中，中期和间期细胞在i(17q)发生率方面存在一些微小的数量差异，并且不同病例之间i(17q)信号大小存在相当大的异质性。我们的结果表明，i(17q)的形成是由于着丝粒近端p臂的断裂，并且不同病例的断点各不相同。
