Bentz M, Döhner H, Cabot G, Lichter P
Deutsches Krebsforschungszentrum, Abt. Organisation komplexer Genome, Heidelberg, Germany.
Leukemia. 1994 Sep;8(9):1447-52.
Fluorescence in situ hybridization (FISH) is a powerful tool for the analysis of chromosomal abnormalities in metaphase and interphase cells. Interphase cytogenetics has become an important technique for the analysis of leukemias, since in many cases it may be difficult to obtain metaphase spreads representative for the malignant clone(s). Using suitable DNA probes, leukemia samples can be screened for the most relevant chromosomal abnormalities. Alternatively, chromosomal imbalances can be identified by applying the new approach of comparative genomic hybridization. In this review, recent advances in the analysis of leukemia made possible by FISH are presented and future prospects of molecular cytogenetics are discussed.
荧光原位杂交（FISH）是分析中期和间期细胞染色体异常的有力工具。由于在许多情况下可能难以获得代表恶性克隆的中期染色体铺片，间期细胞遗传学已成为分析白血病的一项重要技术。使用合适的DNA探针，可以筛查白血病样本中最相关的染色体异常。另外，可通过应用比较基因组杂交的新方法来识别染色体失衡。在本综述中，介绍了FISH在白血病分析中取得的最新进展，并讨论了分子细胞遗传学的未来前景。
