膀胱癌中10号染色体的非随机变化。通过荧光原位杂交技术检测间期细胞核。

Nonrandom changes of chromosome 10 in bladder cancer. Detection by FISH to interphase nuclei.

作者信息

Wang M R, Perissel B, Taillandier J, Kémény J L, Fonck Y, Lautier A, Benkhalifa M, Malet P

机构信息

Laboratoire d'Histologie-Embryologie-Cytogénétique, Faculté de Médecine de Clermont-Ferrand, France.

出版信息

Cancer Genet Cytogenet. 1994 Mar;73(1):8-10. doi: 10.1016/0165-4608(94)90175-9.

DOI:10.1016/0165-4608(94)90175-9

PMID:8174080

Abstract

Fluorescence in situ hybridization (FISH) to interphase nuclei has been a valuable method for examining the chromosome copies in tumor cells in clinical practice. Twelve cases of transitional cell carcinoma (TCC) of the bladder were investigated with a biotin-labeled repetitive DNA probe to detect numerical aberrations of chromosome 10 in interphase nuclei. The cells containing one fluorescent signal were screened in two of seven non-invasive tumors and in four of five invasive tumors. Two patients presented two FISH spots of different sizes. More than two signals were seen in one invasive tumor. The findings suggest that partial or complete loss of a chromosome 10 is a nonrandom aberration in bladder cancer.

摘要

荧光原位杂交（FISH）技术应用于间期核已成为临床实践中检测肿瘤细胞染色体拷贝数的一种重要方法。本研究采用生物素标记的重复DNA探针，对12例膀胱移行细胞癌（TCC）进行检测，以观察间期核中10号染色体的数目畸变情况。在7例非浸润性肿瘤中的2例以及5例浸润性肿瘤中的4例中，发现了含有一个荧光信号的细胞。2例患者出现了两个大小不同的FISH信号点。在1例浸润性肿瘤中观察到了两个以上的信号。这些结果提示，10号染色体的部分或完全缺失是膀胱癌中一种非随机的畸变。