Wang M R, Perissel B, Taillandier J, Malet P
Laboratoire d'histologie-embryologie-cytogénétique, faculté de médecine, Clermont-Ferrand, France.
Bull Cancer. 1994 Dec;81(12):1060-6.
Transitional cell carcinomas of human urinary bladder were studied by interphase fluorescence in situ hybridization (FISH). With current hybridization to isolated nuclei, 26 tumors were investigated and nonrandom +7, -9 and -10 were identified. Monosomy 11, tetraploidies and polyploidies were detected in invasive and poor-differentiated tumors. Hybridization on frozen sections offers another means of analysing surgical samples. FISH to vesical washings can be applied to monitor tumor progression. Hybridizations on paraffine sections and on tissues previously stored in liquid nitrogen allow retrospective studies of the archived materials. Our data suggest that the interphase FISH can become a powerful tool for cytogenetic studies of bladder cancer.
采用间期荧光原位杂交（FISH）技术对人膀胱癌移行细胞癌进行了研究。通过目前对分离细胞核的杂交技术，对26例肿瘤进行了研究，发现了非随机的 +7、-9 和 -10 异常。在浸润性和低分化肿瘤中检测到 11 号染色体单体、四倍体和多倍体。在冰冻切片上进行杂交为分析手术样本提供了另一种方法。对膀胱冲洗液进行 FISH 可用于监测肿瘤进展。在石蜡切片和先前储存在液氮中的组织上进行杂交可对存档材料进行回顾性研究。我们的数据表明，间期 FISH 可成为膀胱癌细胞遗传学研究的有力工具。
