Amiel A, Lishner M, Lalkin A, Gaber E, Manor Y, Fejgin M, Yarkoni S, Ravid M
Department of Medicine, Meir Hospital Kfar-Saba, Israel.
Cancer Genet Cytogenet. 1994 Apr;73(2):165-8. doi: 10.1016/0165-4608(94)90203-8.
Data concerning oncogene activation in CLL are very limited. When studied by Southern blot, rearrangements of bcl-1, bcl-2, and bcl-3 have been only infrequently reported. We evaluated the role of fluorescence in situ hybridization (FISH) in the detection of gene rearrangements in two CLL patients. We used multiple DNA probes, including those of chromosome 12, immunoglobulin heavy and light chains, and the oncogenes bcl-1, bcl-2, and bcl-3. Additionally, routine cytogenetic study was performed. In one patient, trisomy 12 and bcl-2 translocation were demonstrated by both methods, while trisomy 12 and bcl-1 translocation were seen in the second patient, who had a normal karyotype. Larger studies should evaluate the role of FISH in the detection of oncogene involvement in CLL and compare it with other molecular methods.
关于慢性淋巴细胞白血病（CLL）中癌基因激活的数据非常有限。通过Southern印迹法研究时，bcl-1、bcl-2和bcl-3的重排仅偶尔有报道。我们评估了荧光原位杂交（FISH）在两名CLL患者基因重排检测中的作用。我们使用了多种DNA探针，包括12号染色体、免疫球蛋白重链和轻链以及癌基因bcl-1、bcl-2和bcl-3的探针。此外，还进行了常规细胞遗传学研究。在一名患者中，两种方法均检测到12号染色体三体和bcl-2易位，而在第二名核型正常的患者中则发现了12号染色体三体和bcl-1易位。更大规模的研究应评估FISH在检测CLL中癌基因受累情况的作用，并将其与其他分子方法进行比较。
