Hermonat P L
Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences, Little Rock 72205.
Cancer Res. 1994 Apr 15;54(8):2278-81.
Human papillomavirus (HPV) infection, in particular that by HPV type 16, is positively associated with cervical/genital cancer. In contrast, human adeno-associated virus (AAV) infection is negatively associated with these same cancers. AAV has also been found to inhibit the oncogenic properties of a variety of DNA viruses, including bovine papillomavirus type 1, a relative of HPV-16. Taken together, these findings suggested the possibility that AAV and HPV-16 might interact, with AAV inhibiting HPV-16's oncogenic phenotype. Here this hypothesis is addressed using tissue culture assays measuring HPV-16-directed phenotypes. It is found that the cotransfection of AAV type 2 Rep78-positive plasmids resulted in the inhibition of HPV-16 sequence containing plasmids in oncogenic transformation/focus formation, G418-resistant colony formation, and chloramphenicol acetyltransferase expression assays. These data are consistent with the hypothesis that AAV's negative association with cervical cancer is at least partially due to its ability to inhibit HPV-16 expression.
人乳头瘤病毒（HPV）感染，尤其是16型HPV感染，与宫颈癌/生殖器癌呈正相关。相比之下，人腺相关病毒（AAV）感染与这些相同的癌症呈负相关。还发现AAV可抑制多种DNA病毒的致癌特性，包括1型牛乳头瘤病毒，它是HPV - 16的亲属。综上所述，这些发现提示了AAV与HPV - 16可能相互作用的可能性，即AAV抑制HPV - 16的致癌表型。在此，使用测量HPV - 16导向表型的组织培养试验来探讨这一假说。研究发现，2型AAV Rep78阳性质粒的共转染在致癌转化/集落形成、G418抗性集落形成和氯霉素乙酰转移酶表达试验中导致含HPV - 16序列质粒的抑制。这些数据与以下假说一致，即AAV与宫颈癌的负相关至少部分归因于其抑制HPV - 16表达的能力。
