Ono S, Bhargava V, Ono S, Mao L, Hagan G, Rockman H A, Ross J
Department of Medicine, University of California San Diego, La Jolla 92093.
Cardiovasc Res. 1994 Mar;28(3):349-57. doi: 10.1093/cvr/28.3.349.
The aim was to develop a digital video contrast angiographic method for assessing global left ventricular function and volume in vivo in the rat and then to apply it to a study of ventricular remodelling after coronary occlusion, with and without reperfusion.
Digital contrast angiography was performed on 29 rats, including the following groups: sham operated (n = 11), non-transmural myocardial infarction produced by reperfusion (n = 8), and transmural infarction produced by permanent occlusion (n = 10). Under anaesthesia three weeks later, biplane fluoroscopic images were acquired following venous contrast injection. Levophase images were digitised, and left ventricular end diastolic and end systolic volumes and ejection fractions were obtained using an area-length method. Left ventricular ejection fraction data also were calculated by videodensitometry from video density curves.
Compared to the sham operated group, the reperfused group showed a significant decrease in left ventricular ejection fraction, at 53(SD 7) v 70(5)% (p < 0.01), and an increase in end diastolic volume. The permanent occlusion group showed a further decrease in the ejection fraction [40(8)%] and a further significant increase in end diastolic volume compared to the reperfused group (p < 0.01). Left ventricular ejection fraction correlated inversely with percent infarct size (r = 0.882) and showed a positive correlation with the spared epicardial area (r = 0.721). Most haemodynamic variables, including maximum left ventricular dP/dt, failed to discriminate between the groups. The methods showed reasonable accuracy when tested in vitro using contrast filled balloons. In vivo, the left ventricular ejection fraction calculated by densitometry showed adequate interobserver variability (2 SD +/- 8.5 percentage points), but the area-length method showed somewhat more scatter.
Digital video contrast angiography is a feasible method for the assessing global left ventricular function in the rat and should be useful in other small animal models. Significant differences in left ventricular volumes and ejection fractions were detected between reperfused and permanent occlusion groups, whereas haemodynamic variables showed non-significant trends. Reperfusion after 45 min of occlusion caused sparing of the epicardium, prevented unfavourable remodelling, and improved the ejection fraction compared to permanent occlusion.
旨在开发一种数字视频对比血管造影方法，用于在大鼠体内评估左心室整体功能和容积，然后将其应用于冠状动脉闭塞后伴或不伴再灌注的心室重构研究。
对29只大鼠进行数字对比血管造影，包括以下几组：假手术组（n = 11）、再灌注产生的非透壁性心肌梗死组（n = 8）和永久性闭塞产生的透壁性梗死组（n = 10）。三周后在麻醉状态下，静脉注射造影剂后采集双平面荧光透视图像。对左心相图像进行数字化处理，并使用面积-长度法获得左心室舒张末期和收缩末期容积以及射血分数。左心室射血分数数据也通过视频密度测定法从视频密度曲线计算得出。
与假手术组相比，再灌注组左心室射血分数显著降低，分别为53（标准差7）%和70（5）%（p < 0.01），舒张末期容积增加。与再灌注组相比，永久性闭塞组射血分数进一步降低[40（8）%]，舒张末期容积进一步显著增加（p < 0.01）。左心室射血分数与梗死面积百分比呈负相关（r = 0.882），与保留的心外膜面积呈正相关（r = 0.721）。大多数血流动力学变量，包括左心室最大dP/dt，未能区分各组。在体外使用充有造影剂的球囊进行测试时，这些方法显示出合理的准确性。在体内，通过密度测定法计算的左心室射血分数显示观察者间变异度适中（2标准差±8.5个百分点），但面积-长度法显示的离散度稍大。
数字视频对比血管造影是评估大鼠左心室整体功能的一种可行方法，在其他小动物模型中也应有用。再灌注组和永久性闭塞组之间检测到左心室容积和射血分数有显著差异，而血流动力学变量显示出无显著差异的趋势。与永久性闭塞相比，闭塞45分钟后再灌注可使心外膜得以保留，防止不良重构，并改善射血分数。
