Gelbard H A, Applegate C D
Department of Neurology, University of Rochester Medical Center, NY 14642.
Epilepsy Res. 1994 Jan;17(1):23-9. doi: 10.1016/0920-1211(94)90076-0.
Amygdala kindling resulted in significant increases in the expression of D2 receptor mRNA in the nucleus accumbens and striatum 30 days following the last kindling stimulation. Densitometric analyses of tissue sections incubated in the presence of an oligonucleotide probe directed against D2 receptor cDNA indicated a 20-35% increase in D2 receptor mRNA in these regions following kindling. Kindling from the amygdala followed by piriform cortical kindling in the transfer paradigm (overkindling) resulted in significant further increases in D2 receptor mRNA expression in both the accumbens (150% increase) and striatum (120% increase). There were no observed hemispheric asymmetries in D2 receptor mRNA in either kindled or overkindled animals. The data indicate an enduring upregulation of extrapyramidal D2 receptor mRNA following the kindling process. How this change may relate to kindling-induced alterations in seizure susceptibility or behaviors mediated by limbic dopaminergic pathways are questions for future studies.
杏仁核点燃在末次点燃刺激后30天导致伏隔核和纹状体中D2受体mRNA的表达显著增加。在用针对D2受体cDNA的寡核苷酸探针孵育的组织切片的光密度分析表明，点燃后这些区域的D2受体mRNA增加了20%-35%。在转移模式下（过度点燃），先进行杏仁核点燃，然后进行梨状皮质点燃，导致伏隔核（增加150%）和纹状体（增加120%）中D2受体mRNA表达进一步显著增加。在点燃或过度点燃的动物中，未观察到D2受体mRNA的半球不对称性。数据表明点燃过程后锥体外系D2受体mRNA持续上调。这种变化如何与点燃诱导的癫痫易感性改变或由边缘多巴胺能通路介导的行为相关，是未来研究的问题。
