Caruso D, Risé P, Galella G, Regazzoni C, Toia A, Galli G, Galli C
Institute of Pharmacological Sciences, University of Milano, Italy.
FEBS Lett. 1994 May 2;343(3):195-9. doi: 10.1016/0014-5793(94)80554-7.
Deuterated arachidonic acid (AA, [2H8]20:4 n-6) 1-25 microM, is converted to other fatty acids, as evaluated by gas chromatography-mass spectrometry, in THP-1 cells. The major products, in the 1 to 10 microM range, are 22:4 (elongated) and 20:3 (reduced in 5). At 25 microM, 24:4, 24:5 and 22:5 accumulate, with [2H8]/[2H0] ratios higher than in AA. At high AA concentration preferential conversion to elongated fatty acids with 5 unsaturations, through a 6 desaturase takes place and the 4-desaturated 22:5 appears to be formed through beta-oxidation of 24:5.
氘代花生四烯酸（AA，[2H8]20:4 n-6）浓度为1-25微摩尔时，通过气相色谱-质谱分析评估，在THP-1细胞中会转化为其他脂肪酸。在1至10微摩尔范围内，主要产物为22:4（延长型）和20:3（5位减少型）。在25微摩尔时，24:4、24:5和22:5积累，其[2H8]/[2H0]比值高于AA中的比值。在高AA浓度下，通过6-去饱和酶优先转化为具有5个不饱和键的延长型脂肪酸，4-去饱和的22:5似乎是通过24:5的β-氧化形成的。
