通过磷脂酰肌醇特异性磷脂酶C-IV从真核细胞质膜释放碱性磷酸二酯酶I。从豚鼠器官释放。（注：原文中“Cacia porcellus”可能有误，推测为“Cavia porcellus”即豚鼠）

Alkaline phosphodiesterase I release from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C--IV. The release from Cacia porcellus organs.

Nakabayashi T, Matsuoka Y, Ikezawa H, Kimura Y

Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.

Int J Biochem. 1994 Feb;26(2):171-9. doi: 10.1016/0020-711x(94)90142-2.

Abstract

Alkaline phosphodiesterase I release from organs of Cacia porcellus by the action of phosphatidylinositol-specific phospholipase C (PIPLC) of Bacillus thuringiensis was studied. 2. A significant amount of alkaline phosphodiesterase I was released from both slices and homogenate of the kidney and small intestine but not from the liver or pancreas. 3. The release of the enzyme from kidney brush border membranes was dependent on, or proportional to, the reaction time and the PIPLC concentration. The enzyme release by PIPLC was suppressed when the PIPLC was heat-inactivated before addition to the reaction mixture. This suggests that the enzyme release must be due to direct action of PIPLC on kidney brush border membranes. 4. The released alkaline phosphodiesterase I had a molecular weight of 240,000 and was activated by Mg2+, but strongly inhibited by EDTA, thiol reagents and 5'-nucleotide-containing compounds.

摘要

研究了苏云金芽孢杆菌的磷脂酰肌醇特异性磷脂酶C（PIPLC）作用下，从豚鼠器官中释放碱性磷酸二酯酶I的情况。2. 肾脏和小肠的切片及匀浆中释放出大量碱性磷酸二酯酶I，但肝脏或胰腺中未释放。3. 酶从肾刷状缘膜的释放取决于反应时间和PIPLC浓度，或与之成正比。当PIPLC在加入反应混合物之前热失活时，PIPLC介导的酶释放受到抑制。这表明酶的释放一定是由于PIPLC对肾刷状缘膜的直接作用。4. 释放的碱性磷酸二酯酶I分子量为240,000，被Mg2+激活，但受到EDTA、硫醇试剂和含5'-核苷酸的化合物的强烈抑制。