de Agostini A I, Ramus M A, Rosenberg R D
Department of Gynaecology and Obstetrics, Geneva University Hospital, Switzerland.
J Cell Biochem. 1994 Feb;54(2):174-85. doi: 10.1002/jcb.240540206.
The heparan sulfate proteoglycans that bind and activate antithrombin III (aHSPGs) are synthesized by endothelial cells as well as other nonvascular cells. We determined the amounts of cell surface-associated and soluble aHSPGs generated by the rat fat pad endothelial (RFP) cell line and the fibroblast (LTA) cell line. The RFP cells exhibit higher levels of cell surface-associated aHSPGs as compared to LTA cells, whereas LTA cells release larger amounts of soluble aHSPGs as compared to RFP cells. After confluence RFP cells show an increase in both cell surface-associated and soluble aHSPGs. In contrast, postconfluent LTA cells maintain a constant level of cell surface-associated and soluble aHSPGs. These observations indicate that different cell types can preferentially accumulate aHSPGs as cell surface-associated or soluble forms which could reflect alternate biological functions.
结合并激活抗凝血酶III的硫酸乙酰肝素蛋白聚糖（aHSPGs）由内皮细胞以及其他非血管细胞合成。我们测定了大鼠脂肪垫内皮（RFP）细胞系和成纤维细胞（LTA）细胞系产生的细胞表面相关aHSPGs和可溶性aHSPGs的量。与LTA细胞相比，RFP细胞表现出更高水平的细胞表面相关aHSPGs，而与RFP细胞相比，LTA细胞释放出更多的可溶性aHSPGs。汇合后，RFP细胞的细胞表面相关aHSPGs和可溶性aHSPGs均增加。相反，汇合后的LTA细胞的细胞表面相关aHSPGs和可溶性aHSPGs保持恒定水平。这些观察结果表明，不同的细胞类型可以优先将aHSPGs积累为细胞表面相关形式或可溶性形式，这可能反映了不同的生物学功能。
