Horner A A
Department of Physiology, University of Toronto, Ontario, Canada.
Biochem J. 1995 Nov 15;312 ( Pt 1)(Pt 1):245-9. doi: 10.1042/bj3120245.
[35S]Heparin proteoglycans were isolated from the skins and peritoneal mast cells of male rats aged 2 to 22 months. Their [35S]heparin chains were separated on antithrombin-agarose into fractions with high and low affinities for antithrombin. In skin, the proportion of 35S-labelled high-affinity heparin chains declined from 23% at 2 months to 8% at 12 months and did not change significantly between 12 and 22 months. In peritoneal mast cells, the proportion of 35S-labelled high-affinity heparin chains increased from 14% at 2 months to 21% at 4 months and then did not vary significantly until 15 months of age. By 21 months a consistent and significant decline to 8% occurred. The structures of high-affinity heparin proteoglycans did not change with age. Their decreased proportions, without change in their structure, may indicate that they are produced by a unique subset of mast cells, the proportion of which declines with age. [35S]heparan sulphate chains were isolated from skins and brains of rats in the same age range and fractionated on antithrombin-agarose. There were no significant variations in the proportions of 35S-labelled high-affinity heparan sulphate chains in skin (10%) or brain (24%) between 4 and 22 months of age.
从2至22月龄雄性大鼠的皮肤和腹膜肥大细胞中分离出[35S]肝素蛋白聚糖。它们的[35S]肝素链在抗凝血酶琼脂糖上分离成对抗凝血酶具有高亲和力和低亲和力的组分。在皮肤中，35S标记的高亲和力肝素链的比例从2个月时的23%下降到12个月时的8%，在12至22个月之间没有显著变化。在腹膜肥大细胞中，35S标记的高亲和力肝素链的比例从2个月时的14%增加到4个月时的21%，然后直到15月龄时都没有显著变化。到21个月时，一致且显著下降至8%。高亲和力肝素蛋白聚糖的结构不随年龄变化。它们比例的下降，而结构不变，可能表明它们是由肥大细胞的一个独特亚群产生的，其比例随年龄下降。从相同年龄范围的大鼠的皮肤和大脑中分离出[35S]硫酸乙酰肝素链，并在抗凝血酶琼脂糖上进行分级分离。在4至22月龄之间，皮肤（10%）或大脑（24%）中35S标记的高亲和力硫酸乙酰肝素链的比例没有显著变化。
