Tobin D J, Orentreich N, Fenton D A, Bystryn J C
Ronald O. Perelman Department of Dermatology, New York University Medical Center, New York 10016.
J Invest Dermatol. 1994 May;102(5):721-4. doi: 10.1111/1523-1747.ep12375477.
Although alopecia areata is suspected to be an autoimmune disease, no direct evidence of an altered immune response to components of the hair follicle has been reported. We studied whether antibodies to normal human anagen scalp hair follicles are present in individuals with alopecia areata. Thirty-nine alopecia areata sera and 27 control sera were tested by Western immunoblotting for antibodies to 6 M urea-extractable proteins of normal anagen scalp hair follicles. At serum diluted 1:80, all alopecia areata subjects (100%), but only 44% of control individuals, had antibodies directed to one or more antigens of approximately 57, 52, 50, 47, or 44 kD. The incidence of antibodies to individual hair follicle antigens in alopecia areata was up to seven times more frequent than in control sera and their level up to 13 times greater and was statistically significant for all five antigens. Tissue specificity analysis indicated that these antigens were selectively expressed in hair follicles. These findings indicate that individuals with alopecia areata have abnormal antibodies directed to hair follicle antigens, and support the hypothesis that alopecia areata is an autoimmune disease.
尽管斑秃被怀疑是一种自身免疫性疾病，但尚未有关于毛囊成分免疫反应改变的直接证据报道。我们研究了斑秃患者体内是否存在针对正常人生长期头皮毛囊的抗体。通过蛋白质免疫印迹法，检测了39份斑秃患者血清和27份对照血清中针对正常人生长期头皮毛囊6M尿素可提取蛋白的抗体。在血清稀释度为1:80时，所有斑秃患者（100%）均有针对约57、52、50、47或44kD一种或多种抗原的抗体，而对照个体中仅有44%有此类抗体。斑秃患者中针对单个毛囊抗原的抗体发生率比对照血清高7倍，其水平比对照血清高13倍，且这五种抗原的差异均具有统计学意义。组织特异性分析表明，这些抗原在毛囊中选择性表达。这些发现表明斑秃患者体内存在针对毛囊抗原的异常抗体，支持斑秃是一种自身免疫性疾病的假说。
