Nicholson P, Addison C, Cross A M, Kennard J, Preston V G, Rixon F J
Medical Research Council Virology Unit, Institute of Virology, Glasgow, U.K.
J Gen Virol. 1994 May;75 ( Pt 5):1091-9. doi: 10.1099/0022-1317-75-5-1091.
The intracellular distributions of three herpes simplex virus type 1 (HSV-1) capsid proteins, VP23, VP5 and VP22a, were examined using vaccinia virus and plasmid expression systems. During infection of cells with HSV-1 wild-type virus, all three proteins were predominantly located in the nucleus, which is the site of capsid assembly. However, when expressed in the absence of any other HSV-1 proteins, although VP22a was found exclusively in the nucleus as expected, VP5 and VP23 were distributed throughout the cell. Thus nuclear localization is not an intrinsic property of these proteins but must be mediated by one or more HSV-1-induced proteins. Co-expression experiments demonstrated that VP5 was efficiently transported to the nucleus in the presence of VP22a, but the distribution of VP23 was unaffected by the presence of either or both of the other two proteins.
利用痘苗病毒和质粒表达系统检测了单纯疱疹病毒1型（HSV-1）三种衣壳蛋白VP23、VP5和VP22a的细胞内分布。在用HSV-1野生型病毒感染细胞的过程中，所有这三种蛋白主要位于细胞核中，而细胞核是衣壳组装的场所。然而，当在没有任何其他HSV-1蛋白的情况下表达时，尽管如预期的那样VP22a仅存在于细胞核中，但VP5和VP23却分布于整个细胞。因此，核定位不是这些蛋白的固有特性，而是必须由一种或多种HSV-1诱导蛋白介导。共表达实验表明，在存在VP22a的情况下，VP5能有效地转运至细胞核，但VP23的分布不受其他两种蛋白中任何一种或两种同时存在的影响。
