Porkka-Heiskanen T, Tuomisto L, Ylinen M, Stenberg D
Department of Physiology, University of Helsinki, Finland.
Life Sci. 1994;54(22):1719-26. doi: 10.1016/0024-3205(94)00612-1.
Rats were deprived of REM sleep (REMS) for 72 h with the platform method and decapitated in the morning immediately after the deprivation or in the afternoon after having been allowed 5 hours of rebound sleep. The histamine concentrations of the anterior and posterior hypothalamus, the cortex, the hippocampus and the pineal gland were measured, as well as the tele-methylhistamine concentrations of the anterior and posterior hypothalamus. Histamine concentrations were no different after REMS deprivation compared to large platform or dry cage controls, but in the anterior hypothalamus histamine levels increased during rebound sleep only in the REMS deprived rats. tele-Methylhistamine/histamine ratios were higher after 72 h of both REMS deprivation and the large platform treatment compared to dry cage controls, indicating increased histamine utilization during the platform treatment procedure.
采用平台法使大鼠快速眼动睡眠（REMS）剥夺72小时，在剥夺后早晨立即断头，或在给予5小时反弹睡眠后的下午断头。测量下丘脑前部和后部、皮质、海马体和松果体的组胺浓度，以及下丘脑前部和后部的 tele-甲基组胺浓度。与大平台或干燥笼对照组相比，快速眼动睡眠剥夺后组胺浓度没有差异，但仅在快速眼动睡眠剥夺的大鼠中，下丘脑前部的组胺水平在反弹睡眠期间增加。与干燥笼对照组相比，快速眼动睡眠剥夺72小时和大平台处理后，tele-甲基组胺/组胺比值更高，表明在平台处理过程中组胺利用增加。
