Botrè F, Botrè C, Greco A, Data P G, Di Giulio C, Morelli L
Istituto di Merceologia, Università La Sapienza, Rome, Italy.
Neurosci Lett. 1994 Jan 31;166(2):126-30. doi: 10.1016/0304-3940(94)90467-7.
The catalytic activity of carbonic anhydrase (CA) contained in the glomus cells of mammalian carotid bodies has been determined in vitro by a potentiometric method. Experiments performed on whole rabbit carotid bodies have shown a very low variability, in terms of the overall CA activity, among organs belonging to different animals maintained in normoxic conditions. Repeated assays performed on each carotid body have shown a marked decrease of the overall CA activity after the first assay, thus suggesting the presence of at least two different forms of enzyme. Experiments performed on carotid bodies belonging to rabbits maintained in normal, hyperoxic and hypoxic conditions have shown that the overall CA activity follows the sequence: hypoxic > normoxic > hyperoxic, matching with the corresponding physiological activity of the carotid body.
通过电位法在体外测定了哺乳动物颈动脉体球细胞中所含碳酸酐酶（CA）的催化活性。对整个兔颈动脉体进行的实验表明，在常氧条件下饲养的不同动物的器官中，就总体CA活性而言，变异性非常低。对每个颈动脉体进行的重复测定表明，第一次测定后总体CA活性显著下降，因此表明至少存在两种不同形式的酶。对处于正常、高氧和低氧条件下的兔颈动脉体进行的实验表明，总体CA活性遵循以下顺序：低氧＞常氧＞高氧，这与颈动脉体相应的生理活性相符。
