碳酸酐酶同工酶在大鼠颈动脉体中的免疫组织化学定位。
Immunohistochemical localization of carbonic anhydrase isozymes in the rat carotid body.
作者信息
Yamamoto Yoshio, Fujimura Minako, Nishita Toshiho, Nishijima Kazutoshi, Atoji Yasuro, Suzuki Yoshitaka
机构信息
Laboratory of Veterinary Anatomy, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.
出版信息
J Anat. 2003 Jun;202(6):573-7. doi: 10.1046/j.1469-7580.2003.00191.x.
The rat carotid body was immunohistochemically stained for carbonic anhydrase I, II and III (CA-I, CA-II and CA-III). Immunoreactivity for CA-I was distributed in type I cells, type II cells and nerve bundles. Smooth muscle cells and endothelial cells of blood vessels were also strongly stained for CA-I. CA-II immunoreactivity was distinctly positive in type I cells and nerve bundles. Vascular smooth muscle cells were weakly positive, and type II cells were negative for CA-II. CA-III immunoreactivity was identified in type I cells and vascular smooth muscle cells. Our results suggest that carbonic anhydrase isozymes in type I cells play an important role in chemoreception for hypercapnia. Immunoreactivities for CA-I and CA-II in the nerve fibres may participate in the synergic action of carotid sinus nerve between hypoxic and hypercapnic stimuli.
对大鼠颈动脉体进行碳酸酐酶I、II和III（CA-I、CA-II和CA-III）的免疫组织化学染色。CA-I的免疫反应性分布于I型细胞、II型细胞和神经束中。血管的平滑肌细胞和内皮细胞也对CA-I呈强染色。CA-II免疫反应性在I型细胞和神经束中明显呈阳性。血管平滑肌细胞呈弱阳性，II型细胞对CA-II呈阴性。在I型细胞和血管平滑肌细胞中鉴定出CA-III免疫反应性。我们的结果表明，I型细胞中的碳酸酐酶同工酶在高碳酸血症的化学感受中起重要作用。神经纤维中CA-I和CA-II的免疫反应性可能参与了颈动脉窦神经在低氧和高碳酸血症刺激之间的协同作用。
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