Tsai T H, Chen C F
National Research Institute of Chinese Medicine, Taipei, Taiwan.
Neurosci Lett. 1994 Jan 31;166(2):175-7. doi: 10.1016/0304-3940(94)90479-0.
A rapid and sensitive set-up consisting of a microbore HPLC with electrochemical detection coupled with two serial on-line microdialysis systems was described to simultaneously measure endogenous levels of acetylcholine (ACh), choline (Ch), dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), 3-methoxytyramine (3-MT), 5-hydroxytryptamine (5-HT), and 5-hydroxyindoleacetic acid (5-HIAA) in freely moving rats. The validity of the present procedure is demonstrated by analyzing the effect of methamphetamine (2 mg/kg, s.c.) on the increase release of DA, 3-MT, 5-HT, and ACh but without effect in Ch, DOPAC, HVA and 5-HIAA from the striatum of freely moving rat.
本文描述了一种快速灵敏的检测装置，它由一台带电化学检测的微径高效液相色谱仪与两个串联的在线微透析系统组成，用于同时测量自由活动大鼠体内乙酰胆碱（ACh）、胆碱（Ch）、多巴胺（DA）、3,4-二羟基苯乙酸（DOPAC）、高香草酸（HVA）、3-甲氧基酪胺（3-MT）、5-羟色胺（5-HT）和5-羟吲哚乙酸（5-HIAA）的内源性水平。通过分析甲基苯丙胺（2 mg/kg，皮下注射）对自由活动大鼠纹状体中DA、3-MT、5-HT和ACh释放增加的影响，但对Ch、DOPAC、HVA和5-HIAA无影响，证明了本方法的有效性。
