Carruthers C, Baumler A, Neilson A, Pressman D
Cancer Res. 1976 May;36(5):1568-72.
The presence of the azocompounds, p-dimethylaminoazobenzene and 3'-methyl-p-dimethylaminoazobenzene, and p-amino-N-acetyl-N-methylaniline (or their metabolites) bound to components of the liver cells of rats fed a single large dose of each compound has been detected using rabbit antibodies raised against the p-azo-N-acetyl-N-methylaniline hapten in the indirect fluorescent antibody technique. Binding of these antibodies was seen on liver sections from rats fed any one of these compounds. When the anti-p-azo-N-acetyl-N-methylaniline antiserum was absorbed with either liver sediments or cytosol fractions from rats fed p-amino-N-acetyl-N-methylaniline, the antibodies reacting with the liver-bound compounds were removed from the antiserum. Also, absorption of the antiserum with liver sediments or cytosol fractions of rats fed either one of the azocompounds selectively removed all of the antibodies reacting with the livers of rats fed that compound but did not remove other antibodies that were still capable of reacting with liver cells of rats fed the other azocompound or p-amino-N-acetyl-N-methylaniline. Thus this antiserum appears to contain several different anti-p-azo-N-acetyl-N-methylaniline antibodies with different structural requirements for reaction. Some can react with the azocompounds or certain of their metabolites, while others require more of the p-azo-N-acetyl-N-methylaniline structure for reaction. Some of the antibodies appear to react with liver-bound p-dimethylaminoazobenzene but not with liver-bound 3'-methyl-p-dimethylaminoazobenzene, while still others react with 3'-methyl-p-dimethylaminoazobenzene but not with p-dimethylaminoazobenzene.
利用针对对氮偶氮 -N- 乙酰 -N- 甲基苯胺半抗原产生的兔抗体，采用间接荧光抗体技术，已检测到给大鼠单次大剂量喂食对二甲基氨基偶氮苯、3'- 甲基 - 对二甲基氨基偶氮苯和对氨基 -N- 乙酰 -N- 甲基苯胺（或它们的代谢产物）后，这些化合物与大鼠肝细胞成分结合的情况。在喂食这些化合物中任何一种的大鼠肝脏切片上都能看到这些抗体的结合。当用喂食对氨基 -N- 乙酰 -N- 甲基苯胺的大鼠的肝脏沉淀物或胞质溶胶组分吸收抗对氮偶氮 -N- 乙酰 -N- 甲基苯胺抗血清时，与肝脏结合化合物反应的抗体从抗血清中被去除。此外，用喂食任何一种偶氮化合物的大鼠的肝脏沉淀物或胞质溶胶组分吸收抗血清，可选择性地去除与喂食该化合物的大鼠肝脏反应的所有抗体，但不会去除仍能与喂食另一种偶氮化合物或对氨基 -N- 乙酰 -N- 甲基苯胺的大鼠肝细胞反应的其他抗体。因此，这种抗血清似乎含有几种不同的抗对氮偶氮 -N- 乙酰 -N- 甲基苯胺抗体，它们反应的结构要求不同。有些能与偶氮化合物或其某些代谢产物反应，而另一些则需要更多的对氮偶氮 -N- 乙酰 -N- 甲基苯胺结构才能反应。一些抗体似乎与肝脏结合的对二甲基氨基偶氮苯反应，但不与肝脏结合的 3'- 甲基 - 对二甲基氨基偶氮苯反应，而还有一些则与 3'- 甲基 - 对二甲基氨基偶氮苯反应，但不与对二甲基氨基偶氮苯反应。
