Simon-Sarkadi L, Holzapfel W H
Department of Biochemistry and Food Technology, Technical University Budapest, Hungary.
Z Lebensm Unters Forsch. 1994 Mar;198(3):230-3. doi: 10.1007/BF01192600.
An automated ion-exchange chromatographic method is described for determination of biogenic amines in leafy vegetables using a cation-exchange resin column in potassium form and a three-buffer system. The following amines were separated and quantified for Chinese cabbage, endive, iceberg lettuce and radiocchio: putrescine, histamine, cadaverine, spermidine, agmatine, spermine, tyramine. Amines from vegetable samples were extracted with 10% trichloroacetic acid. The total concentration of these amines ranged from 14 to 20 micrograms/g fresh weight. Spermidine was the major polyamine detected, present at concentrations of 7-15 micrograms/g fresh weight.
描述了一种自动离子交换色谱法，该方法使用钾型阳离子交换树脂柱和三缓冲系统测定叶菜类蔬菜中的生物胺。对大白菜、苦苣、卷心莴苣和红菊苣中的以下胺类进行了分离和定量：腐胺、组胺、尸胺、亚精胺、胍丁胺、精胺、酪胺。蔬菜样品中的胺类用10%三氯乙酸提取。这些胺类的总浓度范围为14至20微克/克鲜重。检测到的主要多胺是亚精胺，其浓度为7-15微克/克鲜重。
