Hrabák A, Antoni F, Csuka I
1st Department of Biochemistry, Semmelweis University Medical School, Budapest, Hungary.
Acta Physiol Hung. 1993;81(1):45-57.
The arginase produced by peritoneal macrophages is not synthesized de novo in short-term (3 h) cultures after harvesting the cells. In long-term cultures the arginase synthesis is restored. In contrast to arginase lysozyme is continuously synthesized in short-term cultures. These statements were proved by the following experimental results: 1. Protein synthesis inhibitor and lysosomotropic agents did not alter the arginase level. 2. Arginine and its analogue, canavanine and ornithine were not able to change the arginase activity. 3. The product of an alternative metabolic pathway of arginine, sodium nitrite, did not affect arginase activity. 4. Effectors influencing the synthesis of cyclic nucleotides (cAMP, cGMP), indomethacin, sodium nitroprusside and an analogue of cAMP had no effect on the arginase activity. 5. Arginase activity could not be significantly modified either by an in vitro Micrococcus luteus treatment or by changing the adherence period of peritoneal exudate cells. 6. When arginase was produced in murine peritoneal macrophages at various periods with medium change, the total arginase released into the media from murine and rat macrophages did not exceed the original intracellular arginase content of the adhered cells during the first 6 hours.
腹膜巨噬细胞产生的精氨酸酶在收获细胞后的短期（3小时）培养中并非重新合成。在长期培养中，精氨酸酶的合成得以恢复。与精氨酸酶不同，溶菌酶在短期培养中持续合成。这些说法由以下实验结果得以证明：1. 蛋白质合成抑制剂和溶酶体促渗剂并未改变精氨酸酶水平。2. 精氨酸及其类似物刀豆氨酸和鸟氨酸无法改变精氨酸酶活性。3. 精氨酸的另一条代谢途径的产物亚硝酸钠并不影响精氨酸酶活性。4. 影响环核苷酸（cAMP、cGMP）合成的效应物、吲哚美辛、硝普钠及cAMP类似物对精氨酸酶活性均无影响。5. 体外藤黄微球菌处理或改变腹膜渗出细胞的黏附时间均无法显著改变精氨酸酶活性。6. 当在不同时期更换培养基使鼠腹膜巨噬细胞产生精氨酸酶时，在最初6小时内，从鼠和大鼠巨噬细胞释放到培养基中的总精氨酸酶不超过黏附细胞最初的细胞内精氨酸酶含量。
