Yu X, Raoult D
Unité des Rickettsies, Faculté de Medecine, Marseille, France.
Am J Clin Pathol. 1994 May;101(5):603-6. doi: 10.1093/ajcp/101.5.603.
Twelve hybridoma clones producing monoclonal antibodies to Afipia felis, a putative causative agent of cat scratch disease, were derived from BALB/c mice immunized with A felis. All 12 monoclonal antibodies were species-specific for A felis and reacted with lipopolysaccharide antigens of A felis. These monoclonal antibodies belong to immunoglobulin (Ig) G1, IgG2a, IgG2b, IgG3, and IgM isotypes. All monoclonal antibodies reacted with both agar-grown A felis and tissue culture-propagated A felis. Formalin fixation did not alter the reactivity of the antigen with the monoclonal antibodies. These monoclonal antibodies could be a useful tool for investigation of the disputed role of A felis in cat scratch disease.
从用阿菲披亚菌免疫的BALB/c小鼠中获得了12个产生针对阿菲披亚菌（一种猫抓病的假定病原体）单克隆抗体的杂交瘤克隆。所有12种单克隆抗体对阿菲披亚菌具有种属特异性，并与阿菲披亚菌的脂多糖抗原发生反应。这些单克隆抗体属于免疫球蛋白（Ig）G1、IgG2a、IgG2b、IgG3和IgM同种型。所有单克隆抗体都能与琼脂培养的阿菲披亚菌和组织培养繁殖的阿菲披亚菌发生反应。福尔马林固定不会改变抗原与单克隆抗体的反应性。这些单克隆抗体可能是研究阿菲披亚菌在猫抓病中存在争议作用的有用工具。
