对从孕妇外周血中分离出的滋养层细胞的形态学研究。
A morphologic study of trophoblast isolated from peripheral blood of pregnant women.
作者信息
Hawes C S, Suskin H A, Petropoulos A, Latham S E, Mueller U W
机构信息
Department of Obstetrics and Gynaecology, Flinders University of South Australia, Bedford Park.
出版信息
Am J Obstet Gynecol. 1994 May;170(5 Pt 1):1297-300. doi: 10.1016/s0002-9378(94)70144-x.
OBJECTIVE
Our purpose was to evaluate the morphologic characteristics of cells separated from peripheral maternal blood with immunomagnetic beads coated with trophoblast-reactive monoclonal antibodies of restricted specificity.
STUDY DESIGN
Blood samples were collected from 14 pregnant women at 9 to 12 or 16 to 18 weeks' gestation. Immunomagnetic beads coated with trophoblast-reactive monoclonal antibodies were used to isolate cells from the blood. The isolated cells were then fixed, embedded, and sectioned to enable morphologic identification.
RESULTS
Multinucleate cells identical to syncytiotrophoblast sprouts were identified in 12 of 14 samples.
CONCLUSION
Multinucleate syncytiotrophoblast can be isolated from the peripheral blood of normal pregnant women in both the first and second trimesters of pregnancy. The isolated trophoblast may allow prenatal genetic diagnosis by a noninvasive technique.
目的
我们的目的是评估用包被有特异性受限的滋养层反应性单克隆抗体的免疫磁珠从孕妇外周血中分离出的细胞的形态学特征。
研究设计
在妊娠9至12周或16至18周时从14名孕妇采集血样。使用包被有滋养层反应性单克隆抗体的免疫磁珠从血液中分离细胞。然后将分离出的细胞固定、包埋并切片以进行形态学鉴定。
结果
在14个样本中的12个中鉴定出了与合体滋养层芽相同的多核细胞。
结论
在妊娠的第一和第二孕期均可从正常孕妇外周血中分离出多核合体滋养层。分离出的滋养层可能允许通过非侵入性技术进行产前基因诊断。
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