Bertero M T, Camaschella C, Serra A, Bergui L, Caligaris-Cappio F
Dipartimento di Scienze Biomediche ed Oncologia Umana, Università di Torino, Italy.
Prenat Diagn. 1988 Oct;8(8):585-90. doi: 10.1002/pd.1970080806.
A syncytiotrophoblast-associated antigen identified by the monoclonal antibody (McAb) H315 is detectable on the surface of a low proportion of peripheral blood cells in pregnant women, raising the possibility of a new approach to prenatal diagnosis of genetic disorders. We aimed at verifying the trophoblastic origin of H315+ cells and their use for prenatal diagnosis of beta-thalassaemia. H315+ cells were separated from the peripheral blood of pregnant women: the DNA obtained from these cells in two selected cases was shown to have genetic markers indistinguishable from those of the mother and definitely different from the fetus. Our results suggest that H315 antigen is expressed by maternal cells and that prenatal diagnosis on peripheral blood of the mother using H315 McAb is not feasible.
一种由单克隆抗体（McAb）H315识别的合体滋养层相关抗原，在少数孕妇外周血细胞表面可检测到，这为遗传疾病的产前诊断提供了一种新方法的可能性。我们旨在验证H315+细胞的滋养层来源及其在β地中海贫血产前诊断中的应用。从孕妇外周血中分离出H315+细胞：在两个选定病例中，从这些细胞中获得的DNA显示出与母亲的遗传标记无法区分，且与胎儿的遗传标记明显不同。我们的结果表明，H315抗原由母体细胞表达，使用H315单克隆抗体对母亲外周血进行产前诊断是不可行的。
