Pool C, Aplin J D, Taylor G M, Boyd R D, Donnai P
Department of Obstetrics and Gynecology, St. Mary's Hospital, Manchester, England.
Am J Reprod Immunol. 1989 Jul;20(3):77-80. doi: 10.1111/j.1600-0897.1989.tb00643.x.
In view of its potential importance in antenatal diagnosis of genetic disease, we have used flow cytometry to attempt to enumerate trophoblast cells in the peripheral blood of pregnant females. As a trophoblast marker we have used the monoclonal antibody (MAB) H315, which reacts with placental-type alkaline phosphatase (PLAP) associated with the cell-membrane of the syncytiotrophoblast. We analyzed blood leucocytes from 62 pregnant females between 6 and 22 weeks' gestation, 26 nonpregnant females, and 21 males. Overall, we detected more H315+ cells in pregnant than in nonpregnant female blood but, because of the wide variation in both groups, we found no statistically significant difference between them. We were unable to detect H315+ cells in 25% of pregnant females within the resolution of the technique. There was, in contrast, a significant difference between the number of H315+ cells in pregnant females and males. Because we were unable to demonstrate other trophoblast markers on H315+ cells isolated from maternal peripheral blood, the origin of these cells is unclear. This implies that these cells may be an unsuitable source of material for antenatal diagnosis.
鉴于其在遗传病产前诊断中的潜在重要性，我们运用流式细胞术试图对怀孕女性外周血中的滋养层细胞进行计数。作为滋养层标记物，我们使用了单克隆抗体（MAB）H315，它与合体滋养层细胞膜相关的胎盘型碱性磷酸酶（PLAP）发生反应。我们分析了62名妊娠6至22周的怀孕女性、26名未怀孕女性以及21名男性的血液白细胞。总体而言，我们在怀孕女性血液中检测到的H315 +细胞比未怀孕女性的多，但由于两组数据差异较大，我们发现两者之间无统计学上的显著差异。在该技术的分辨率范围内，我们在25%的怀孕女性中未能检测到H315 +细胞。相比之下，怀孕女性和男性体内H315 +细胞的数量存在显著差异。由于我们无法在从母体外周血分离出的H315 +细胞上证明其他滋养层标记物，这些细胞的来源尚不清楚。这意味着这些细胞可能不是产前诊断的合适材料来源。
