Cohn L A, Kinnula V L, Adler K B
Department of Anatomy, Physiological Sciences, and Radiology, North Carolina State University College of Veterinary Medicine, Raleigh 27606.
Am J Physiol. 1994 Apr;266(4 Pt 1):L397-404. doi: 10.1152/ajplung.1994.266.4.L397.
Guinea pig tracheal epithelial (GPTE) cells in primary air/liquid interface culture were exposed to H2O2, and the rate of H2O2 consumption by apical and basolateral surfaces was measured. GPTE cells had potent H2O2 scavenging ability, with faster consumption of H2O2 from the apical surface. Inhibition of catalase (Cat) with sodium azide (NaAz) significantly attenuated the ability of GPTE cells to remove higher concentrations of H2O2. Depletion of reduced glutathione, the substrate for glutathione peroxidase (GPO), with DL-buthionine-[S,R]-sulfoximine (BSO) did not affect consumption of H2O2. Dissolution of mucus from the cells reduced scavenging activity of the cultures and basement membrane/extracellular matrix material (BM/ECM) deposited by the cells demonstrated significant H2O2-scavenging activity. The results suggest that GPTE cells retain antioxidant capability in vitro when cultured in an air/liquid interface. This capacity to scavenge H2O2 appears to rely on Cat, as well as on mucus and BM/ECM material. However, a significant amount of H2O2 scavenging appears to depend on other, yet unidentified, antioxidant system(s).
将原代气液界面培养的豚鼠气管上皮（GPTE）细胞暴露于过氧化氢中，并测量其顶侧和基底外侧表面消耗过氧化氢的速率。GPTE细胞具有强大的过氧化氢清除能力，且从顶侧表面消耗过氧化氢的速度更快。用叠氮化钠（NaN₃）抑制过氧化氢酶（Cat）可显著减弱GPTE细胞清除较高浓度过氧化氢的能力。用DL-丁硫氨酸-[S,R]-亚砜亚胺（BSO）耗尽谷胱甘肽过氧化物酶（GPO）的底物还原型谷胱甘肽，并不影响过氧化氢的消耗。去除细胞表面的黏液会降低培养物的清除活性，而细胞沉积的基底膜/细胞外基质材料（BM/ECM）表现出显著的过氧化氢清除活性。结果表明，GPTE细胞在气液界面培养时在体外保留了抗氧化能力。这种清除过氧化氢的能力似乎依赖于Cat，以及黏液和BM/ECM材料。然而，大量的过氧化氢清除似乎依赖于其他尚未明确的抗氧化系统。
