Romarís M, Villena J, Molist A, Heredia A, Bassols A
Departament de Bioquímica i Biologia Molecular, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Biochem Biophys Res Commun. 1994 Apr 29;200(2):925-32. doi: 10.1006/bbrc.1994.1538.
ts371 KiMuSV-transformed rat kidney fibroblasts growing at the permissive temperature to allow the expression of a transforming p21 ras protein secrete a different pattern of chondroitin/dermatan sulfate proteoglycans than when growing at the non-permissive temperature. Transformed cells produce less versican and larger amounts of biglycan and decorin than their normal counterpart, and they do not secrete 35S-labeled PG-100. Western blot experiments showed the presence of PG-100 epitope, suggesting that the apparent effect of the oncoprotein is due to a reduced sulfation of the molecule. Transformed and non-transformed cells are similarly sensitive to the proteoglycan-induction effect of TGF-beta.
ts371 KiMuSV转化的大鼠肾成纤维细胞在允许温度下生长，以允许转化型p21 ras蛋白表达，其分泌的硫酸软骨素/硫酸皮肤素蛋白聚糖模式与在非允许温度下生长时不同。与正常细胞相比，转化细胞产生的多功能蛋白聚糖较少，而双糖链蛋白聚糖和核心蛋白聚糖的量较多，并且它们不分泌35S标记的PG - 100。蛋白质印迹实验显示存在PG - 100表位，这表明癌蛋白的明显作用是由于该分子硫酸化减少。转化细胞和未转化细胞对TGF-β的蛋白聚糖诱导作用同样敏感。
