Szöllösi M S, Borsuk E, Szöllösi D
I.N.R.A., Unité de Biologie de la Fécondation, Station de Physiologie Animale, Jouy-en-Josas, France.
Mol Reprod Dev. 1994 Feb;37(2):146-56. doi: 10.1002/mrd.1080370205.
Nucleate and anucleate fragments of parthenogenetically activated mouse oocytes, as well as cybrids obtained by fusion of anucleate fragments (cytoplasts) of maturing and activated matured oocytes were fertilized at different time after activation. Remodelling of the sperm nucleus was studied by electron microscopy at 1.5 and 3 h after fertilization and, in addition, at 14 h in cybrids. Results show that 1) the nuclear envelope of the sperm nucleus can break down when the insemination takes place after the end of M-phase, but the capacity of the parthenote cytoplasm to remodel the sperm nucleus is restricted in time. 2) Male chromatin can decondense within the old, unbroken nuclear envelope, but in such cases formation of a male pronucleus, one of the two nuclei of zygote possessing inactive nucleoli, is never observed.
孤雌激活的小鼠卵母细胞的有核和无核片段，以及通过成熟和激活的成熟卵母细胞的无核片段（细胞质体）融合获得的胞质杂种，在激活后的不同时间进行受精。受精后1.5小时和3小时，以及胞质杂种在14小时时，通过电子显微镜研究精子核的重塑。结果表明：1）当在M期结束后进行授精时，精子核的核膜可以破裂，但孤雌胚细胞质重塑精子核的能力在时间上受到限制。2）雄性染色质可以在完整的旧核膜内解聚，但在这种情况下，从未观察到雄原核的形成，雄原核是合子的两个核之一，具有无活性的核仁。
