Powell C T, Fair W R, Heston W D
Urologic Oncology Research Laboratory, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
Cell Growth Differ. 1994 Feb;5(2):143-9.
Adenocarcinoma of the prostate is the second leading cause of cancer deaths in men. The protein kinase C (PKC) family of signal transducing kinases has been implicated in neoplastic transformation and progression in other tissues, and some evidence suggests roles for PKC in prostate growth and neoplasia. We have detected expression of eight specific PKC isozyme mRNAs (alpha, beta, gamma, delta, epsilon, eta, theta, and zeta) in normal rat whole prostate and found some of these to be differentially expressed in certain Dunning R-3327 rat prostatic adenocarcinoma sublines. PKC zeta mRNA was detected in normal prostate and Dunning H tumor, whereas an alternatively spliced form of PKC zeta RNA was found in Dunning G tumor and normal brain. Both forms of PKC zeta RNA were markedly reduced in the androgen insensitive, highly metastatic Dunning AT-3, MAT-Lu, and MAT-LyLu tumors. We have cloned and report the sequence of the novel portion of the alternatively spliced form of PKC zeta RNA, which is polyadenylated and present in cytoplasm.
前列腺腺癌是男性癌症死亡的第二大主要原因。信号转导激酶的蛋白激酶C（PKC）家族与其他组织的肿瘤转化和进展有关，并且一些证据表明PKC在前列腺生长和肿瘤形成中发挥作用。我们在正常大鼠全前列腺中检测到了八种特定PKC同工酶mRNA（α、β、γ、δ、ε、η、θ和ζ）的表达，并发现其中一些在某些邓宁R-3327大鼠前列腺腺癌亚系中差异表达。在正常前列腺和邓宁H肿瘤中检测到了PKCζmRNA，而在邓宁G肿瘤和正常脑中发现了PKCζRNA的一种可变剪接形式。在雄激素不敏感、高转移性的邓宁AT-3、MAT-Lu和MAT-LyLu肿瘤中，两种形式的PKCζRNA均显著减少。我们已经克隆并报告了PKCζRNA可变剪接形式的新部分序列，该序列是多聚腺苷酸化的且存在于细胞质中。
