Lyman S D, James L, Johnson L, Brasel K, de Vries P, Escobar S S, Downey H, Splett R R, Beckmann M P, McKenna H J
Department of Molecular Genetics, Immunex Research and Development Corp, Seattle, WA 98101.
Blood. 1994 May 15;83(10):2795-801.
Using a fragment of the murine flt3 ligand as a probe, we have succeeded in cloning a human flt3 ligand from a human T-cell lambda gt10 cDNA library. The human and murine ligands are 72% identical at the amino acid level. Analysis of multiple cDNA clones shows that alternative splicing of the human flt3 mRNA can occur at a number of positions. A recombinant soluble form of the human flt3 ligand stimulates the proliferation and colony formation of a subpopulation of human bone marrow cells that are CD34+ and are enriched for primitive hematopoietic cells. In addition, the human flt3 ligand also stimulates the proliferation of cells expressing murine flt3 receptors. Northern blot analysis shows widespread expression of flt3 ligand mRNA transcripts in human tissues.
我们以小鼠fms样酪氨酸激酶3（flt3）配体的一个片段作为探针，成功地从人T细胞λgt10 cDNA文库中克隆出了人flt3配体。人源和鼠源配体在氨基酸水平上有72%的同源性。对多个cDNA克隆的分析表明，人flt3 mRNA可在多个位置发生可变剪接。重组可溶性人flt3配体可刺激人骨髓中一群CD34+且富含原始造血细胞的亚群细胞的增殖和集落形成。此外，人flt3配体还可刺激表达鼠源flt3受体的细胞的增殖。Northern印迹分析显示，flt3配体mRNA转录本在人体组织中广泛表达。
