Gadient R A, Otten U
Department of Physiology, University of Basel, Switzerland.
Brain Res. 1994 Feb 21;637(1-2):10-4. doi: 10.1016/0006-8993(94)91211-4.
Using RT-PCR, the development profile of interleukin-6 (IL-6) and its receptor (IL-6R) mRNAs in rat brain was investigated. Our results indicate that IL-6 and IL-6R mRNAs are coexpressed and are developmentally regulated in a tissue-specific manner. Highest levels of both transcripts were detected in the adult hippocampus. Most pronounced developmental changes of IL-6 message levels were observed in the rat striatum increasing up to 8-fold. By contrast, in all other regions such as neocortex, hippocampus, cerebellum and pons/medulla oblongata only minor changes (2- to 3-fold) in IL-6 expression were seen. In most tissues IL-6 mRNA levels peaked at day 20. Marked induction of the receptor message levels was detected in the striatum, hippocampus and neocortex (8- to 10-fold) whereas no changes were observed in the cerebellum and the pons/medulla oblongata. The expression pattern of both genes in various brain areas during postnatal development strongly supports the concept of IL-6 as a candidate for a new neurotrophic factor.
运用逆转录聚合酶链反应（RT-PCR），研究了大鼠脑中白细胞介素-6（IL-6）及其受体（IL-6R）mRNA的发育情况。我们的结果表明，IL-6和IL-6R mRNA共表达，并以组织特异性方式受到发育调控。在成年海马体中检测到这两种转录本的最高水平。在大鼠纹状体中观察到IL-6信息水平最显著的发育变化，增加了8倍。相比之下，在新皮层、海马体、小脑和脑桥/延髓等所有其他区域，IL-6表达仅出现轻微变化（2至3倍）。在大多数组织中，IL-6 mRNA水平在第20天达到峰值。在纹状体、海马体和新皮层中检测到受体信息水平的显著诱导（8至10倍），而在小脑和脑桥/延髓中未观察到变化。出生后发育过程中这两个基因在不同脑区的表达模式有力地支持了IL-6作为一种新型神经营养因子候选者的概念。
