Shizuya K, Komori T, Fujiwara R, Miyahara S, Ohmori M, Nomura J
Department of Psychiatry, Mie University School of Medicine, Tsu, Japan.
Life Sci. 1997;61(10):PL 135-40. doi: 10.1016/s0024-3205(97)00608-5.
Using the reverse transcriptase-polymerase chain reaction (RT-PCR), we investigated the influence of restraint stress on the expression of the mRNA for interleukin-6 (IL-6) and the mRNA for the IL-6 receptor (IL-6R) in the rat brain. After rats had been restrained for 4 hours, the hypothalamus and midbrain were removed at fixed intervals up to 24 hours, and levels of IL-6 mRNA and of IL-6R mRNA in these regions were determined by RT-PCR. Restraint stress significantly enhanced the expression of IL-6 mRNA and reduced that of IL-6R mRNA in the midbrain, whereas the stress caused the reduced expression of IL-6R mRNA without any change in the level of IL-6 mRNA in the hypothalamus. After the stress, the expression of mRNAs for IL-6 and IL-6R continued to diminish in both regions. These findings indicate that the levels of mRNAs for both of IL-6 and IL-6R in the rat brain can be influenced by restraint stress.
我们使用逆转录聚合酶链反应（RT-PCR），研究了束缚应激对大鼠脑内白细胞介素-6（IL-6）的mRNA和IL-6受体（IL-6R）的mRNA表达的影响。大鼠被束缚4小时后，在长达24小时的固定间隔时间处死，取出下丘脑和中脑，通过RT-PCR测定这些区域中IL-6 mRNA和IL-6R mRNA的水平。束缚应激显著增强了中脑IL-6 mRNA的表达并降低了IL-6R mRNA的表达，而该应激导致下丘脑IL-6R mRNA表达降低，IL-6 mRNA水平无任何变化。应激后，两个区域中IL-6和IL-6R的mRNA表达均持续下降。这些发现表明，大鼠脑中IL-6和IL-6R的mRNA水平可受束缚应激影响。
