Gadient R A, Otten U
Department of Physiology, University of Basel, Switzerland.
Neurosci Lett. 1993 Apr 16;153(1):13-16. doi: 10.1016/0304-3940(93)90065-s.
Using the sensitive technique of reverse transcription followed by polymerase chain reaction (RT-PCR), the expression of interleukin-6 (IL-6) and its receptor (IL-6R) in rat hypothalamus was investigated. Our results show a differential expression of the mRNAs for IL-6 and IL-6R during postnatal development: whereas IL-6 mRNA slightly declines with increasing age, IL-6R mRNA levels markedly increase (4-fold). The time course of these changes correlates with the maturation of the hypothalamo-pituitary-adrenocortical axis, indicating an involvement of the IL-6 system in neuroendocrine regulation. Expression of both genes in other brain areas suggest additional functions in the central nervous system (CNS).
采用逆转录聚合酶链反应（RT-PCR）这种灵敏技术，研究了白细胞介素-6（IL-6）及其受体（IL-6R）在大鼠下丘脑的表达情况。我们的结果显示，在出生后发育过程中，IL-6和IL-6R的mRNA存在差异表达：随着年龄增长，IL-6 mRNA略有下降，而IL-6R mRNA水平显著增加（4倍）。这些变化的时间进程与下丘脑-垂体-肾上腺皮质轴的成熟相关，表明IL-6系统参与神经内分泌调节。这两种基因在其他脑区的表达提示它们在中枢神经系统（CNS）中具有额外功能。
